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By Karl Maramorosch

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25 mM, was a mediator of HCG synthesis. Moreover, the concentrations of hydroxyurea t h a t were ineffective by themselves in promoting HCG production could potentiate the effects of sodium butyrate as shown in Fig. , 1977). , 1980). In this experiment, concentrations of hydroxyurea alone, butyrate alone, and the two drugs together t h a t inhibited cell multiplication to approximately the same extent were studied with respect to the level of induction of HCG. As shown in Table VIII, sodium butyrate is more effective as an inducer t h a n is hydroxyurea.

V. , 1975). Butyrate is a potent inhibitor of HeLa cell proliferation 46 RODY P. , 1977). Since this inhibition of DNA synthesis may be related to the ectopic induction of glycopeptide hormone synthesis in HeLa cells, the effects of other well-charàcterized inhibitors of DNA synthesis were studied. Hydroxyurea, a drug t h a t blocks the entry of cells into S-phase of the cell cycle, mediated increased HCG production in the HeLa lines studied. 25 mM, was a mediator of HCG synthesis. Moreover, the concentrations of hydroxyurea t h a t were ineffective by themselves in promoting HCG production could potentiate the effects of sodium butyrate as shown in Fig.

48 HOURS AFTER ADDITION FIG. 6. Effect of sodium butyrate on proliferation of HeLa cell populations. Replicate 25-cm2 plastic flasks were harvested at the indicated times after addition of inhibitor. Ranges of cell counts on duplicates from two experiments are indicated by the vertical bars except where the range is smaller than graphic symbols. · · , No additions (control); Δ Δ, 1 mM butyrate; A A, 5 mM butyrate; ♦ ♦, 1 mM hydroxyurea (Fallon, and Cox, 1979). index). Table IX compares the time course of entry in S phase of HeLa cells grown with 5 m M butyrate compared to asynchronous control cultures and to cells grown with 1 m M hydroxyurea, a wellcharacterized inhibitor of DNA synthesis t h a t is known to focus cells in early S phase (Pfeiffer and Tolmach, 1967).

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